![]() Prepare a 5µg/ml solution of anti-CD3ε (clone 145-2C11) in sterile PBS.96-well flat-bottom tissue culture plates with lids (e.g., Costar® Cat.Sterile, single-cell suspension (e.g., splenocytes, lymph node cells), isolated T cells or T cell subsets.Cell culture medium (e.g., RPMI-1640 or IMDM supplemented with 10% FBS and 2mM L-glutamine). ![]() Anti-mouse CD3ε, clone 145-2C11 (Ultra-LEAF™ format, Cat.T Cell Activation with anti-CD3 Antibodies Protocol - Mouse
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